BPCL-FICL15 Flow Injection Chemiluminescence Analyzer Introduction: The BPCL-FICL15 flow injection chemiluminescence analyzer is a research-oriented flow injection chemiluminescence detection platform, consisting of a flow injection system and a chemiluminescence detection system. The flow injection system comprises three 3-channel peristaltic pumps, a six-way rotary valve, and a standardized flow cell. The chemiluminescence detection system utilizes a high-sensitivity photomultiplier tube (PMT) as the detector, with adjustable parameters such as amplification factor, acquisition rate, and sampling time. All pipelines are made of standard 1/16 PTFE tubing and 1/4 standard connectors, enabling continuous sample injection and intermittent sample injection using a quantitative loop. It can also be used in conjunction with other equipment to form a comprehensive chemiluminescence detection system. Related applications: 1. Real-time online analysis of chemiluminescence 2. Chemiluminescence timing sampling analysis 3. ATP and fluorescein detection 4. Free radical/reactive oxygen species analysis 5. Analysis of environmental water quality samples 6. Coupling with liquid chromatography Technical Specifications: 1. Ultra-weak light detection system (1) Detector: photomultiplier tube, photon pulse measurement. (2) Detection limit: It can provide a counting rate of 10,000-20,000 counts per second for a weak light source of 10^-13W. (3) Long-term stability of the instrument: RSD ≤ 1.5%, tested using a standard light source. (4) Wavelength range: 300-650 nm (5) Instrument counting analysis range: covering five orders of magnitude (6) Sampling interval: 0.01-10000 ms, adjustable by software.. (7) Light window specification: Diameter 44 mm (8) High-voltage output: negative (0-1500) V, current output: 2 mA. The negative high-voltage output value can be set through software, and it also features a high-voltage feedback function, enabling automatic software regulation with an accuracy of 0.1V. 2. Sample room (1) Specifications of the sample chamber: Length × Width × Height: 304 × 147 × 58 mm (2) Static sample temperature control: up to 65 ℃, temperature control accuracy: 0.5 ℃. (3) Static injection sample cup: made of quartz, with diameters of 15mm and 26mm respectively. (4) Piping and wiring connectors: 2 reducers (5-12mm) on each of the left and right panels, 2 1/16 pipe connectors on each of the left and right panels, and 3 1/16 pipe connectors on the front panel. (5) Static sample injection port: 1. (6) The door of the sample chamber is equipped with an automatic sensor. If the door is not properly closed, the light window shutter cannot be opened to prevent accidental damage to the photomultiplier tube due to misoperation. (7) The light window shutter in the sample chamber is an automatic shutter. When detection begins, the light window shutter automatically opens, and when detection ends, it automatically closes. This ensures that the detector can open the sample chamber door without closing the shutter. At the same time, when the light intensity exceeds the range or there is light leakage protection, the light window automatically closes to prevent damage to the photomultiplier tube from strong light. 3. Analysis software (1) Communication mode: USB, with a maximum communication rate of 480 Mbit/s, and plug-and-play functionality. (2) Software Interface: Settings for parameters such as high-pressure output, temperature control, acquisition rate, manual and automatic switching of the two-position six-way rotary valve, and peristaltic pump flow rate. It features automatic background subtraction, automatic range adjustment for acquisition count rate, real-time display of data point dynamic curves, and real-time display of acquisition time, count rate, and total count. (3) Raw data: Optional formats include EXCEL and TXT, with automatic or manual saving options. (4) Analysis platform: It can display data as point plots, line charts, and curve graphs, with the ability to zoom in, zoom out, and pan the coordinates. It also features curve overlay, curve smoothing, curve fitting (exponential, logarithmic, polynomial, etc.), Gaussian function fitting (Gaussian), Lorentz function fitting (Lorentz), Fourier transform, manual peak search, manual integration, etc. Additionally, it can regenerate new raw data and save it. 4. Chemiluminescence flow cell (1) Material of flow cell: quartz material (2) Light transmittance: Both sides are translucent (3) Processing technology: lithography (4) Supporting pipeline: outer diameter of 1/16 inch, inner diameter of 1 mm, made of PTFE (5) Connector: 1/4-28UNF, made of PTFE (6) Flow channel shape: Y-shaped with 2 inlets, U-shaped coiled, and 1 outlet (7) Flow channel specifications: width 1mm, length 176mm, cross-sectional area: 0.48mm² (8) Supporting Fixtures: Supporting chip protection fixtures, as well as chemiluminescence holders 5. Two-position six-way rotary valve (1) Valve head material: Polyetheretherketone (PEEK) (2) Switching mode: manual switching or automatic program-controlled switching. For automatic program-controlled switching, gradient switching time can be set through the program control list. (3) Connector specification: 1/4-28 UNF 6. Peristaltic pump (1) Number of channels: 3 channels (2) Number and material of rollers: 6 rollers, made of stainless steel (3) Card material: POM (4) Suitable hose specifications: Inner diameter: 0.13-3.2 mm, wall thickness: 0.8-1 mm (5) Standard hose and flow range: a. Inner diameter: 1 mm, wall thickness: 1 mm, flow range: 0.006-6 mL/min b. Inner diameter: 2 mm, wall thickness: 1 mm, flow range: 0.02-20 mL/min c. Inner diameter: 2.4 mm, wall thickness: 0.8 mm, flow range: 0.03-30 mL/min
BPCL-FICL15 Flow Injection Chemiluminescence Analyzer Introduction: The BPCL-FICL15 flow injection chemiluminescence analyzer is a research-oriented flow injection chemiluminescence detection platform, consisting of a flow injection system and a chemiluminescence detection system. The flow injection system comprises three 3-channel peristaltic pumps, a six-way rotary valve, and a standardized flow cell. The chemiluminescence detection system utilizes a high-sensitivity photomultiplier tube (PMT) as the detector, with adjustable parameters such as amplification factor, acquisition rate, and sampling time. All pipelines are made of standard 1/16 PTFE tubing and 1/4 standard connectors, enabling continuous sample injection and intermittent sample injection using a quantitative loop. It can also be used in conjunction with other equipment to form a comprehensive chemiluminescence detection system.