Introduction : Principle : The plate is placed on the rotary table of the screw inoculator , and after the sample to be tested is taken up by the sample injector , the agar surface of the culture dish center is contacted , and meanwhile , the sample needle moves from the center of the dish dish to one side , and the bacterial suspension is inoculated to the agar surface . After inoculation , the cultured colonies are distributed according to the spiral distribution of the archimedean spiral and are distributed more and more with the increase of the radius . Spiral plate method : the sample is inoculated onto the plate by using a screw inoculation instrument . After inoculation , the colonies are distributed on the spiral track , and the distribution of the colonies becomes thinner and thinner with the increase of the radius . A special counting grid is adopted to count the colonies on the flat plate from the periphery of the flat plate to the center , so that the number of microorganisms in the sample can be obtained . The spiral vaccination method was recorded in the FDA , AOAC ( American Association of Analytical Chemists ) , APHA ( American Public Health Association ) , AFNOR ( French Standards Consortium ) VO8 - 100 , ISO 7218 , GB 4789 , 28 - 2013 ( Quality Requirements for Food Microbiology Testing - Media and Reagents ) , SN / T 2098 - 2008 ( Colony Count Test Method in Food and Cosmetic ) - Spiral Plate Method ) . Field of application : The invention is suitable for detecting the total number of bacteria in a sample with more bacteria , such as a government detection institution , a food , a medicine and a cosmetic industry . Microbial research units , users who need a large amount of gradient to dilute the flat plates every day . Medium quality control , such as culture medium production enterprise , large food , medicine , cosmetics enterprise . Technical parameters : 1 . Environmental requirements : temperature : 5 - 40 ℃ , humidity : 20 % -90 % ; Power requirements : 110 - 240 V , 50 Hz . 2 . The bacteria content can be detected quantitatively from 30 - 4 × 105cfu / ml ( 9cm plate ) or 30 - 4 × 106 cfu / ml ( 15cm plate ) by using only one flat plate , the number of colonies on the plate is good , the edge sterility of dish is easy to count , and the result is accurate ; 3 . The core of Quickprepare is microprocessor controlled inoculation device , according to one key , from sucking sample , screw inoculation , disinfection solution and water washing , fully automatic completion ; 4 , Adopting Teflon inoculation needle , no residual sample , unique inoculation needle balancing device , and automatically processing plates with different agar thicknesses ; the agar plates are not scratched ; 5 , The logarithmic mode and the linear mode can be selected , and various inoculation amounts can be selected under different modes according to the number of the sample bacteria ; 6 . The capacity of the precision syringe is 100μL ;The typical inoculation time was 8 s at the inoculation volume of 50.mu . l . 7 . The lowest detection limit of the theory is 2.5cfu / ml ( 400μL linear mode ) ;The maximum error is less than 5 cfu / ml when the number of colonies is 400 cfu / ml . Powerful microprocessor , software automatic control , sample processing and vaccination are completed automatically , and the plate with different agar thickness can be automatically processed by using the inoculator algorithm . 8 . The trajectory of sample inoculation is archimedean screw , and has one or log two inoculation modes . After inoculation with 9cm plate , a 1000 - fold dilution effect can be produced . For 15cm plate , a 10000 - fold dilution effect can be produced . 9 . Loading the sample , inoculating the plate and cleaning the inoculation head can be completed by one key . No consumables , especially inoculating needles , etc . are required , and no subsequent cost is generated . 10, Cleaning liquid storage system can complete automatic cleaning cycle, convenient to prepare the next sample, to ensure the continuity of operation and improve efficiency. 11. By detecting the movement and relative position of the inoculum head, the inoculum holder and the turntable, the data obtained by the microprocessor are compared with the factory setting to produce accurate correction data. 12 , The shell of the host machine is a corrosion - resistant polyurethane , the control key adopts a color touch screen , and the parameter can be set to be selectable and locked into two modes ; 13 . Automatic cleaning system , one - button cleaning , disinfecting twice with disinfectant and washing with sterile water twice , and backflushing when the inoculation needle is blocked ;the disinfectant uses sodium hypochlorite or alcohol ; 14 . The screw inoculation has both manual and automatic modes , which can directly extract the sample from the non - standard container ; Several plates can be continuously inoculated with a single sample . 15 . An inoculation station of adjustable size can be selected for use in a variety of different sizes of plates .
