MGISEQ-T7 gene sequencer Introduction
The MGISEQ-T7 is an ultra-high-throughput next-generation sequencer with the world's first "four-chip platform" that supports 1-4 chips ready to go/off automatically, and each chip runs independently and flexibly. The PE150 runs at full load for less than 24 hours, and the image data processing speed is 15 times higher than the previous products. The daily flux is as high as 6T, which is the current global high-throughput genetic sequencer. Features
High accuracy
The MGISEQ-T7 sequencing library uses the rolling circle amplification technique to prepare DNB, which enables library amplification PCR-Free. The DNB preparation process always uses the original single-stranded DNA as a template for isothermal amplification, and the single amplification error does not accumulate, which greatly reduces the original sequence information of the genome, and has a significant advantage in Indel detection.
Low repeat rate
Based on the preparation of PCR-Free DNB, the MGISEQ-T7 chip follows the Pattern Array technology. Each activation site is equivalent to the size of DNB. The DNB is uniformly arranged and uniformly adsorbed. The fluorescence signal released by DNB during sequencing can be accurately captured. The Duplicate Rate of output data is on average less than 3%, which is better than the industry average.
Low tag jump
The unique library construction technology and rolling circle amplification technology of Huada Zhixing Sequencing Platform make the index Hopping probability much lower than other sequencing platforms. The probability of Index Hopping can be controlled from 0.0001% to 0.0004% with only one Barcode. MGISEQ-T7 supports single and double barcode sequencing methods, which is very suitable for the mixing of large numbers of samples. Technical Parameters
Sequencing speed: PE150<24 hours
Detection repeatability: depending on the specific application, the repeat sequence rate is on average less than 3%
Detection accuracy: Q30 ≥ 85%
Flux: 6T
Reading range: SE50, PE100, PE150
Introduction
The MGISEQ-T7 is an ultra-high-throughput next-generation sequencer with the world's first "four-chip platform" that supports 1-4 chips ready to go/off automatically, and each chip runs independently and flexibly. The PE150 runs at full load for less than 24 hours, and the image data processing speed is 15 times higher than the previous products. The daily flux is as high as 6T, which is the current global high-throughput genetic sequencer.
