Brief introduction: ClearFirst-2000Prime protein purification system is one key protein purification system developed by Shanghai flicker biologic development. It is mainly used to replace the AKTAprime plus protein purification system of GE company in the United States. It is mainly used in the protein purification work of small life science laboratory. Compared with the traditional method, the efficiency of purification work is greatly improved and the efficiency of the purification is greatly improved. The amount of manpower and material resources. The performance of ClearFirst-2000Prime protein purification system in pump, detector, distillate collector and control workstation software surpasses the AKTAprime plus protein purification system of GE company. It has very powerful function and high performance price ratio. It is an ideal choice for the protein purification laboratory of our country. Instrument characteristics: 1, The use of eight way valve for buffer selection and sample injection, has very high practical value. 2, The use of high-precision constant current pump, small pulsation, stable baseline, accurate flow output, performance is much higher than the peristaltic pump system; 3, The flow rate is 0-10 ml/min, and the analysis and preparation can be upgraded to 0-50ml/min. 4, The full wavelength detector can be used to select any wavelength between 190-850 nm. 5, The automatic fraction collector eliminates the problem of pipe jumping, disorder and overflow, and has high stability. 6, The X-Y matrix collector has no motor at the bottom, and the safety is better than that of the disc fraction collector. 7, A high quality gradient curve can be formed and the performance is much higher than that of the gradient cup communicant system. 8, High precision distillate collection software ensures the accurate matching of test tube and peak position, and finds convenience. 9, A three-way valve with waste liquid is collected, which can be collected only after high and medium pressure. 10, Accurate collection of peaks, avoid omission and cross contamination, and save manpower; 11, With peak collection, time, level, manual collection and other modes, high flexibility. 12, The flow cell can use different optical path to adapt to different analysis and purification requirements. 13, With the method database, preset different purification methods can be called at any time, and one key completes automatic operation. 14, The principle and structure of a large range and high precision special conductivity detector are the same as that of imported products. 15, The PH detector has the function of temperature correction, and its accuracy is better than that of imported products. 16, Compact size, very easy to put equipment into chromatography freezer. 17, The system pressure piping is basically PEEK material, good pressure and biocompatibility. 18, The use of multi way valve sampling way, accurate sampling, to avoid air into the system; 19, The instrument can be fully controlled by computer, which greatly reduces the difficulty of using. 20, Self checking, user management and sample management can be used to connect LIMS information system. 21, The fraction collection test tube can reach 120, and the number of distilled fractions is better than that of imported products. 22, Using grating monochromator, the wavelength accuracy is better than 0.2nm, and the bandwidth is better than the filter product. 23, The workstation is full of Chinese interface and convenient to use. 24, Upgrading only a few components can be upgraded to a high pressure system with a system pressure up to 3000psi. 25, The instrument can be controlled by panel, tablet, notebook computer and desktop computer. 26, The instrument can be combined with the high-speed countercurrent system for the purification of natural products. 27, The instrument can use G-10 analysis column for automatic analysis of antibiotic macromolecule impurities. 28, The hardware configuration and software function of the instrument are better than that of AKTAprime protein purification system. 29, This instrument fraction collector can change different collection quantities by switching different test tubes. 30, This instrument supports all unmanned operation after pump sampling and manual sampling. Instrument parameters: Solvent compatibility: water buffers commonly used for purification of biomolecules. Velocity range: 0.01-50ml/min; Pressure range: 0-1MPa (10bar, 145psi) Viscosity: up to 10cP; Wavelength range: 190-850nm; Diameter length: 2mm; The range of electrical conductivity: 1uS/cm-999.9mS/cm; PH determination range: 0-14; PH measurement accuracy: 0.01PH unit, temperature compensation; Test tube load: 120 (15-18mm); Use of instrument: 1, Protein purification (gel, ion exchange, hydrophobic interaction, affinity and other methods); 2, The separation and purification of nucleic acid; 3, The separation and purification of macromolecule impurities in antibiotics; 4, The separation and purification of natural products;
