Introduction
By constructing a "super-trace volume (fL-pL) electrochemical reaction module", the device introduces macroscopic bio/electrochemical measurement theory and method into single-cell analysis for the first time, and measures single cells and single cells in real time by electrochemically analyzing reaction products. Chemical information such as biomolecular activity/structure in organelles. The instrument breaks through the technical bottleneck of single cell analysis and reduces the difficulty of analysis; it has the characteristics of strong versatility, high throughput, high degree of automation, high temporal and spatial resolution, real-time and in-situ detection; Features
1. Accurate, ultra-micro: non-mechanical injection/extraction technology, with the advantages of loading, less sampling, more precise control, and the minimum loading is flying.
2. Minimally invasive operation: minimally invasive injection (probe tip outer diameter ≤ 200nm), minimal damage to cells, to ensure cell viability.
3. No retention, no backflow: instantaneous start/stop injection or extraction process, the dead volume is less than the mechanical pump delivery mode, there is no retention or reflow phenomenon.
4. Injection extraction dual function: One device is compatible with both injection and extraction functions, and can share one control channel.
5. Blockage detection: It has online jam detection function. Technical Parameters
Injection/extraction volume: 10-15 (flying)-10-9L (nanoliter)
Minimum injection/extraction volume: 1×10-15L (flying)
Injection flow rate: 0.1-100×10-18L/s (can be adjusted according to experimental requirements)
Micro-extraction space: <500nm region in living cells, including cell microstructure (axons, dendrites, pseudopods, etc.)
Real-time monitoring of the injection/extraction process
Temperature measurement range: -10 ° C ~ 75 ° C
Maximum relative humidity: 80% relative humidity at 31°C (88°F), then linearly reduced
Power supply: 220V, 50/60HZ
Signal data acquisition and analysis software: real-time online analysis and processing of collected images and electrical information data
Introduction
By constructing a "super-trace volume (fL-pL) electrochemical reaction module", the device introduces macroscopic bio/electrochemical measurement theory and method into single-cell analysis for the first time, and measures single cells and single cells in real time by electrochemically analyzing reaction products. Chemical information such as biomolecular activity/structure in organelles. The instrument breaks through the technical bottleneck of single cell analysis and reduces the difficulty of analysis; it has the characteristics of strong versatility, high throughput, high degree of automation, high temporal and spatial resolution, real-time and in-situ detection;
