Product description: The advanced cell culture device, specially designed membrane culture bottle, is very suitable for the culture of antibodies and protein products. The latest high density cell culture device. This design greatly increases the density of the product when the antibody and protein products are cultured on a small scale. Conventional in vitro or in vivo cell culture methods are difficult, often resulting in very low cell density and require effective purification to obtain products. CELLine bioreactor addresses these three limitations when culturing cells in static culture bottles. Wheaton's CELLine bioreactor adopted the technique of cell compartment and medium compartment semi-permeable membrane isolation and culture, which broke the restriction of air, nutrients and metabolic inhibitory factors on cell growth in traditional cell culture. It simulates the environment in which cells grow in the organism to the greatest extent. The aim of high density cell culture and high concentration product expression was achieved. Features: 1. Cell concentration in the cell culture chamber of high-density cell culture is usually 107-108/mL, which is two orders of magnitude higher than that of normal static cell culture technology. 2. High product concentration. Thanks to high cell density, cell product concentration in CELLine culture is 1-5mg/mL, which is 50-100 times higher than that in traditional culture methods 3. Long-term continuous culture.Second chamber culture of CELLine. Users can recycle the product and replace the medium. To achieve the goal of long-term continuous cultivation. 4. Convenient operation. CELLine micro cell factory is easy to operate. Without any adjustment to the original culture system, CELLine can be easily used for culture Reduce downstream processing. 5.The two-compartment culture technique allows both the cell concentration and the product concentration to increase by about two orders of magnitude compared with the ordinary culture. The concentration of the culture itself can even be directly followed up, rather than through the complex separation operation of the ordinary culture. 6. High cost performance. High yield per unit, CELLine use is more cost effective than traditional T bottle and multi-layer culture board. Technical parameters: Inoculation density and collection density: CELLine 350 Preculture (living cells) : 8 x 106 Inoculation volume (mL) : 5 Inoculation concentration (number of living cells /mL) : 1.5x106 Collection concentration (number of living cells /mL) : 107-108 Potency (mg/mL) : 1-5 Monthly antibody yield (mg) : 10-100.
